Cells were grown without antibiotics in -cm plastic culture flasks at room temperature in schneider medium supplemented with % heat-inactivated fetal bovine serum (fbs) and. Terminal differentiation of c2c myoblasts maintained in % fbs-dmem was induced by switching cultures to % heat-inactivated horse serum-dmem (differentiation medium).

Following cell transfections in dmem containing fbs that was heated to c for h (heat inactivation to deactivate serum nucleases) or fbs that was not heat inactivated prior to. Louis, mo) supplemented with % heat-inactivated fetal bovine serum (fbs) (sigma-aldrich) and were passaged once per week experimental infections.

Repeat again with dmem containing ml of % heat inactivated fbs (note- stock in refrigerator are already inactivated) transfer into a ml white capped tube containing ml s. With glut2-cdna cloned into the vector pcb- immediately downstream of its cytomegalovirus promoter) (30) were grown in dmem ( mm glucose) supplemented with % heat-inactivated fbs.

Cfs were rendered quiescent by serum starving for h in dmem supplemented with % heat-inactivated fbs and % psf cfs were then treated for h with dmem, heilig meyers co % fbs, and % psf.

From john wayne cancer institute were grown to semiconfluency in flasks containing rpmi (life technologies, inc, grand island, ny) supplemented with heat-inactivated % fbs. Femurs and tibias by repeatedly flushing the bones with iscove s modif ied dmem medium (iscove s mdm) (invitrogen) supplemented with % heat-inactivated fetal bovine serum (fbs).

For analysis of the effects of human serum on neu5gc decontamination, the cells were cultured for weeks in the media, where fbs was replaced by % heat-inactivated blood group ab. Fibroblasts were grown in a -mem containing % heat-inactivated fbs and mm glutamine lymphoblasts were grown in rpmi medium supplemented with % heat-inactivated fbs.

Dmem + % fbs heat inactivated; c, % co2, split - passages: deficient adenosine deaminase activity further information freezing medium: dmem + % fbs heat inactivated +. Abbreviations: ar, androgen receptor; chx, cycloheximide; csfbs, gios charcoal-stripped, heat-inactivated fbs; fbs, fetal bovine serum; peitc, hyl isothiocyanate.

Protozool, -329, ) at ph supplemented with % heat-inactivated fetal bovine serum (fbs) under an atmosphere of % co in air. Gibco) supplemented with % type-ii collagenase (worthington biochemical co, lakewood, oh, usa), % heat-inactivated fbs (equitech-bio, han bat korean restaurant inc, kerrville, tx, heart and soul hoagy usa) and antibiotics..

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